46 SEED TESTING INTERNATIONAL www.seedtest.org TRAINING AND EDUCATION • Denmark) showed how digital microscopy cannot only be used to store imaged-based seed information, but also to work together on seed identification on the same seed sample, in real time at different locations worldwide. This opens the way to sharing expertise and working together from different locations in a laboratory setting. Finally, Bram Wiggers (Track32, The Netherlands) showed the use of mobile telephone-based computer vision apps for large-scale uniformity and usability counts of seedlings in trays. After the lectures there were several demonstrations of advanced equipment for imaged-based seed testing. The participants attended the demonstrations in small groups in a rotating scheme, allowing for easy discussions and specific questions. Before the joint dinner many participants tried a refreshing swim in the Faxe Bugt. On the second day, participants started the hands-on work in MSI analysis: from exploratory image analysis to use in OSD and purity. The practical work contained exercises to explore and learn different aspects of MSI analysis. MSIs from different seeds were used to illustrate the process from image to discrimination and identification of different species, broken seeds, as well as inert matter. Instructions and assistance were given by a large expert team (Birte Boelt, Marting Himmelboe, Jens Michael Carstensen, Cathrine Underbjerg Hansen, Karsten Hartelius), ensuring support to all participants on an individual basis. The hands-on work was followed by a lecture from Lars Wadsö (University of Lund, Sweden) on the use of calorimetry in seed quality determination. As a social event everybody walked from the workshop location to the Stevns Klint experience, a 30-minute walk along the coastline. At Stevns Klint, traces of the asteroid that hit the earth and wiped out the dinosaurs are still visible. This dramatic moment in Earth’s history is present in a thin layer of clay that helped make Stevns Klint a UNESCO World Lecture topics ranged from an introduction to ISTA, the ISTA Technical Committees (Nadine Ettel, left), the Advanced Technologies Committee, multispectral imaging, RGB imaging, X-ray CT scanning and calorimetry (Lars Wadsö, right), to artificial intelligence applications Participants did hands-on exercises on multispectral image analysis in other seed determination; image analysis specialists helped and instructed them to ensure everybody was able to understand and execute the exercises Different types of equipment were demonstrated to participants in small groups Heritage site. The participants enjoyed the guided tour of the museum as well as the coastal scenery. Before the official workshop dinner, a short presentation (by Bert van Duijn) and interactive discussions were held on the use of AI chatbots in seed testing practice. Examples in the design of seed germination tests and seed test result analysis with the use of AI were presented. In addition, we discussed the policies present in the different work environments regarding the use of AI in seed testing practice. ATC will use the outcome of the discussions for further analysis of the possibilities, limitations, positive and negative aspects of the use of AI tools in seed testing. On the final day, excursions to two seed companies were organised. By bus, the participants first visited DLF at Nørre Alslev. They enjoyed a tour through the grass and clover seed processing facilities. At these facilities MSI analysis in the grass seed processing line is in operation. Samples are analysed by MSI before and during the seed processing to adjust the seed cleaning and sorting process in an optimal way. The demonstration of MSI in operation showed that the application of MSI in seed quality determination is not a future possibility but an already implemented practice. The next visit was to Vikima Seed in Holeby. Vikima Seed has developed an MSI tool for OSD analysis of spinach seeds with very high accuracy. Brit Malec (Vikima Seeds) explained the [long] route to establishment and implementation of the MSI tool in the seed production process. It was impressive to learn that more than 50 % of the world’s spinach seed production is analysed by just one MSI analysis machine. Besides knowledge transfer and practical activities, the networking and discussions among participants were an important part of the workshop. In the discussions on the requirements for new technologies for seed testing, it was concluded that speed, accuracy, repeatability and cost are very important.
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