b'FEATURE ARTICLE Table 1. Techniques used for detection of seed pests in the ISTA seed health methods of the International Rules for Seed Testing (ISTA Rules)ISTA seedHost Pest1 Technique2 TechniqueFirst Lasthealthapplicable toversion modifiedmethod treated seeds37-001a Daucus carota Alternaria dauci Blotter no 2003 20257-001b Daucus carota Alternaria dauci Agar (MA) no 2003 20257-002a Daucus carota Alternaria radicina Blotter no 2003 20257-002b Daucus carota Alternaria radicina Agar (MA) no 2003 20257-003 Helianthus annuus Botrytis cinerea Blotter no 2001 20247-004 Brassica spp. Leptosphaeria maculans Blotter, Agar (MA) no 2001 2025Plenodomus biglobosus7-005 Pisum sativum Ascochyta pisi Agar (MA) no 2000 20257-006 Phaseolus vulgaris Colletotrichum lindemuthianum Paper towels no 2001 20247-007 Linum usitatissimum Alternaria linicola Agar (MA, PDA) no 2014 2024Botrytis cinerea Colletotrichum lini7-008 Picea engelmanniiCaloscypha fulgens Agar (WA) no 2001 2024Picea glauca7-009 Pinus spp.Fusarium circinatum Agar (PDA, DCPA, SNA) no 2001 2024Pseudotsuga menziesii7-010 Oryza sativa Bipolaris oryzae Blotter no 2000 20247-011 Oryza sativa Pyricularia oryzae Blotter no 2000 20247-012 Oryza sativa Trichoconiella padwickii Blotter no 2000 20247-013a Hordeum vulgare Ustilago nuda Embryo extraction yes (chemical) 2001 20257-013b Hordeum vulgare Ustilago nuda Dehulling + Embryo extraction no 2010 20247-014 Triticum aestivum Parastagonospora nodorum Agar (MA, PDA) no 2001 20247-015 Festuca spp.Epichlo coenophialaImmunoblot no 2003 2024Lolium spp. Neotyphodium lolii7-016 Glycine max Phomopsis complex Agar (acidified PDA) no 2003 20257-019a Brassica spp. Xanthomonas campestris pv. campestrisAgar (mFS, mCS20ABN, YDC)no 2003 2025Xanthomonas campestris pv. raphani Molecular, Pathogenicity test7-019b Brassica spp. Xanthomonas campestris pv. campestris Agar (mFS, mCS20ABN, YDC)yes (physical,2014 2025Molecular, Pathogenicity test chemical)7-020 Daucus carota Xanthomonas hortorum pv. carotae Agar (mKM, mD5A, mTBM, YDC)no 2005 2025Molecular, Pathogenicity test7-021 Phaseolus vulgaris Xanthomonas phaseoli pv. phaseoliAgar (MT, XCP1, YDC)no 2006 2025Xanthomonas citri pv. fuscans Molecular, Pathogenicity test7-022 Triticum spp. Microdochium nivale Agar (MA, PDA) no 2007 2024Microdochium majus7-023 Phaseolus vulgaris Pseudomonas savastanoi pv. phaseolicola Agar (MT, mSP, Kings B)no 2007 2025Pathogenicity test7-024 Pisum sativum Pea early-browning virus ELISA no 2007 2025Pea seed-borne mosaic virus7-025 Oryza sativa Aphelenchoides besseyi Morphobiometry no 2007 20247-026 Cucurbits Squash mosaic virus ELISA no 2007 2025Cucumber green mottle mosaic virusMelon necrotic spot virus7-027 Hordeum vulgare Pyrenophora teres Filter paper no 2011 2024Pyrenophora graminea7-028 Solanum lycopersicum Tobacco mosaic virus Indexing yes (physical,2011 2024Tomato mosaic virus chemical)7-029 Pisum sativum Pseudomonas syringae pv. pisi Agar (KBBCA, SNAC), Pathogenicity test no 2014 20257-030 Valerianella locusta Acidovorax valerianellae Grow-out, Molecular yes (physical,2015 2024chemical)7-031 Medicago sativaDitylenchus dipsaci Morphobiometry, Molecular yes (physical) 2018 2024Vicia faba Ditylienchus gigas7-032 Spinacia oleracea Verticillium dahliae Agar (NP-10), Blotter yes (physical,2018 2024chemical)7-033 Cicer arientinum Ascochyta rabiei Agar (MA, PDA), Pathogenicity test no 2025 n/a7-034 Solanum lycopersicum Fusarium oxysporum f.sp. lycopersici Agar (MA, PDA) no 2026 n/a1Names of pests are listed as currently described in the ISTA seed health methods. They are not all aligned with the current taxonomy.2Types of agar include malt agar (MA), potato dextrose agar (PDA), water agar (WA), dichloran chloramphenicol peptone agar (DCPA), Spezieller nhrstoffarmer agar (SNA), modified Fieldhouse Sasser (mFS) medium, yeast dextrose chalk (YDC) agar, modified KM-1 (mKM) medium, modified D-5 agar (mD5A) medium, modified Xanthomonas campestris pv. phaseoli (XCP1) medium, Milk Tween (MT) medium, modified sucrose peptone (mSP) medium. ELISA = enzyme-linked immunosorbent assay.3Definition of treatment: any process, physical, biological or chemical, to which a seed lot is subjected, including seed coatings. ISTA Rules 7.2.3. For details, refer to the ISTA method content.10 SEED TESTING INTERNATIONAL www.seedtest.org'