26 I EUROPEAN SEED I EUROPEAN-SEED.COM B reeders typically spend a lot of their time and efforts in developing new plant varieties that have more disease resistance genes to protect them from harmful pathogens. These traits are very important for farmers and growers and contribute in many cases to environmental aims. When these new plant varieties are ready to be commercialized, it is of course important that such disease resistances are officially assessed to guarantee the resistance claim to the farmers and growers who are buying the seeds. Recently, there has been some controversy over the mandatory testing need of certain disease resistance characteristics. European Seed sat down with Sergio Semon, Expert for Vegetable species at the Community Plant Variety Office (CPVO) and asked him to describe how DUS examination works, which types of disease resistance characteristics there are in testing of plant varieties for Distinctness, Uniformity and Stability (DUS) and how the CPVO solved the controversy. EUROPEAN SEED (ES): DUS TESTING IS DONEONPHENOTYPE,INCLUDINGFOR DISEASERESISTANCECHARACTERISTICS. DO YOU THINK DISEASE RESISTANCE CHARACTERISTICS SHOULD APPEAR IN PBR TEST GUIDELINES? SERGIO SEMON (SFS): The CPVO DUS test protocols are not only applicable to tests for EU and national plant variety protection in EU Member States but also to tests carried out to grant market access under the seed marketing legislation. The protocols contain a table of characteristics, which will result in the phenotypical fingerprint of the candidate variety. There are obvious characteristics such as leaf length, fruit colour, time of maturity, etc. Then for some species, there are also some disease and insect resistance characteristics which are also tested. The creation of disease and insect resistant varieties is an important breeding goal of several seed companies. It is also important for farmers and growers to know whether a certain variety is resistant to a disease or insect. For this reason, the DUS test protocol should reflect these breeding advances. Not only can new varieties be declared distinct based solely on their disease and insect resistance profile, but the information as to whether a variety is resistant or not can also prove to be invaluable in the design of the DUS trial. Since most of the disease and insect resistance characteristics in the DUS test protocol are qualitative (the resistance is either absent or present), such characteristics are highly discriminatory and as a result can greatly reduce the number of comparison varieties grown out in the DUS trial. This makes for a more focused and cost-effective technical examination. ES: ARE THERE DIFFERENCES BE- TWEEN CROP GROUPS USING SUCH CHARACTERISTICS? AND IF SO, WHY IS THAT? SFS: There is active breeding for disease and insect resistance in all four crop sectors (agricul- ture, fruit, ornamentals, vegetable); nonetheless, it is the vegetable sector that has wholeheartedly taken on board the use of such characteristics for WHY IT MATTERS Mandatory disease resistance testing can be a significant burden to smaller breeders, especially when the disease does not appear in their region. A thorough discussion among experts and a phase-in period provide the solution. WHY DO I NEED TO TEST FOR THIS? HOW THE CPVO SOLVED THE CONTROVERSY OVER MANDATORY DISEASE RESISTANCE TESTING. BY: MARCEL BRUINS Sergio Semon