When Goldilocks tromped around in the three bears’ cottage, she was on to something: finding what fits just right matters.
Until six years ago, researchers looking to accelerate their genomic programs had two options for outsourced genotyping. They could choose single-plex (qPCR) genotyping, which looks at a very small number of SNPs. Or, they could choose the far other side of the genotyping spectrum: high-density arrays, or chip-based sequencing, which look at a very large number of SNPs from a low number of samples. For many, single-plex provides too little data to be useful, yet high-density can be far too big in both data produced and cost. Remember Goldilocks? Finding what fits just right matters.
In 2014, we opened Agriplex Genomics with the goal of bridging the gap between low- and high-density genotyping. Our next generation sequencing technology allows us to provide a focused, simultaneous analysis of a mid-range number of markers (as few as 50 or as many as 3,500) from any number of DNA samples.
How do we do it? Our amplicon-based sequencing technology, which we call PlexSeq™, allows us to screen a custom multiplex across thousands of samples simultaneously via a single run of a sequencer. Then, our Plexcall™ software automatically compiles the SNP calls then analyzes the frequency.
PlexSeq™ is compatible with crude DNA extraction methods, accommodates varying starting materials (seed, leaf, roots, etc), and requires only minute DNA concentrations (<4ng per μl). The simple protocol means PlexSeq™ is easy to scale up to large production, is ultra-flexible (SNPs can be added to or omitted from any multiplex), and – because it boasts very low lab consumables – is eco-friendly too. With over 55K unique sequencing barcodes available, PlexSeq™ can handle almost an unlimited number of samples. Its automated analysis means that customers receive a consolidated, actionable report instead of juggling rolls of raw data.
Admittedly, we don’t suit everyone. We don’t focus on the low density marketplace, nor do we strive to provide the same glut of data as the high-density market. Our goal has never been to provide a deep dive into one-off SNPs or to serve intensive breeding programs. Rather, our mission is to provide flexibility and clear return on investment to mid-range crop and livestock breeding customers.
Interested in learning more? We’d love to chat.
No, really: we’d actually love to chat.
Because we’re a comparatively small company, we’re able to invest in every customer interaction. That commitment continues from start to finish through every customer relationship. We take the time to understand your business and develop a solid grasp of your unique needs before you send us your samples. We adjust to best suit your needs: if you don’t have the lab capacity for DNA extraction on-site, we can accept raw tissue samples and handle extraction from our end. We value your time: we guarantee results in four to six weeks but can in certain cases shorten that to as little as two weeks if your breeding program is under time pressure. And we’re committed to making sure your results suit you: when your samples’ analysis is complete, and we send you your consolidated report, expect a follow-up phone call to ensure the results are what you need. Afterall, our business works best if it fits you just right.
Increased data return, lower cost, faster turn-around, topnotch customer service: that’s Agriplex Genomics.
